| Singles competition |
| 32 | Kate Lee (Washington University) def. Gaby DeLeon (Allegheny College) 6-1, 6-1 |
| 32 | Natalie Rahrig (Hope College) def. Lynn Kelly (Ohio Northern women) 6-3, 7-5 |
| 32 | Maria Franco (Kalamazoo College) def. Ceylan Can (Kenyon College) 7-6, 6-2 |
| 32 | Lisa Chionis (Washington University) def. Ali Bush (DePauw University) 6-1, 6-4 |
| 32 | Madeline Hart (Denison) def. Travis, Adrienne (University of Chicago) 6-4, 6-0 |
| 32 | Jayde Robinson (Baldwin Wallace) def. Christina Lombardi (Saint Mary's College), walkover |
| 32 | Taylor LaBarge (Hope College) def. Sarah Hughes (Oberlin College), walkover |
| 32 | Amanda Showalter (DePauw University) def. Grace Winslow (Kenyon College) 6-2, 6-1 |
| 32 | ILANA Blackwood (Kenyon College) def. Schaffer, Caitlyn (Augustana) 6-4, 3-6, 10-5 |
| 32 | Laura Hanselman (Kalamazoo College) def. Claudia Olaes (Oberlin College) 6-1, 2-1, retired |
| 32 | Ellison, Daryn (University of Chicago) def. Madisyn Rini (John Carroll) 6-2, 4-6, 10-7 |
| 32 | Joy Li (College of Wooster) def. Sydney Bergman (Otterbein University) 7-5, 6-0 |
| 32 | Mayada Audeh (Oberlin College) def. Quincey Glupker (Hope College) 7-5, 6-4 |
| 32 | Leah Newhof (Calvin College) def. Amanda Ress Liere (Allegheny College) 6-4, 6-0 |
| 32 | Megan Hickey (Denison) def. Sanjna Tripathy (Washington University) 6-1, 6-3, 11-9 |
| 32 | Ashley Ochsenhirt (Ohio Northern women) def. Angell, Molly (Millikin) 6-1, 6-3 |
| 16 | Kate Lee (Washington University) def. Natalie Rahrig (Hope College), walkover |
| 16 | Lisa Chionis (Washington University) def. Ceylan Can (Kenyon College) 6-0, 6-2 |
| 16 | Jayde Robinson (Baldwin Wallace) def. Madeline Hart (Denison) 6-3, 6-2 |
| 16 | Amanda Showalter (DePauw University) def. Taylor LaBarge (Hope College), walkover |
| 16 | ILANA Blackwood (Kenyon College) def. Laura Hanselman (Kalamazoo College) 4-6, 6-1, 10-7 |
| 16 | Joy Li (College of Wooster) def. Ellison, Daryn (University of Chicago), walkover |
| 16 | Leah Newhof (Calvin College) def. Mayada Audeh (Oberlin College) 7-5, 6-1 |
| 16 | Sanjna Tripathy (Washington University) def. Ashley Ochsenhirt (Ohio Northern women) 6-2, 3-6, 12-10 |
| QF | Kate Lee (Washington University) def. Lisa Chionis (Washington University) 3-6, 6-4, 10-4 |
| QF | Amanda Showalter (DePauw University) def. Madeline Hart (Denison) 6-4, 6-2 |
| QF | ILANA Blackwood (Kenyon College) def. Joy Li (College of Wooster) 6-2, 6-1 |
| QF | Sanjna Tripathy (Washington University) def. Leah Newhof (Calvin College), walkover |
| SF | Kate Lee (Washington University) def. Amanda Showalter (DePauw University) 6-3, 7-5 |
| SF | ILANA Blackwood (Kenyon College) def. Sanjna Tripathy (Washington University) 6-3, 6-2 |
| CH | ILANA Blackwood (Kenyon College) def. Kate Lee (Washington University) 4-6, 6-4, 10-7 |